Analysis Note

ControlBrain tissueCultured neurons

Application

Immunohistochemistry: 1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.Western blot: SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-AP20). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Western blot: A 1:500 dilution was used in a previous lot.Optimal working dilutions must be determined by end user.

Detect MAP2A using this Anti-MAP2A Antibody, AP20 validated for use in IH & WB.

Research CategoryNeuroscience

Research Sub CategoryNeuronal & Glial MarkersNeurofilament & Neuron Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984).

MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).

Immunogen

Bovine MAP2

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB3418

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide

Unpurified

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MAP 2 on 10 µg of PC12 lysates.

Specificity

MAP2a & b. Does not recognize other variants.

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~ 300 kDa